Zeiss LSM 710 Confocal Microscope

Microscope Overview

The Zeiss LSM710 laser-scanning confocal microscope is able to generate high-resolution images of fluorescent specimens. It is especially suited for live cell imaging of relatively thick samples (up to approx. 100 ÁM). Its scanning unit has a spectral recycling loop, two sensitive PMT detectors and a filter-free spectral separation module that can be continuously set over the entire wavelength range. It is equipped with lasers from violet to far red (405, 458, 488, 514, 561 and 633 nm excitation wavelengths). It has a motorized stage capable of tiling, multi-point mark, and find memory function. Current stage holders will support slides, circular glass-bottom culture dishes, and well plates with glass-coverslip bottoms. It is also equipped with an environmental chamber and full stage incubator for precise temperature and CO2 control. The purchase of the Zeiss LSM 710 confocal microscope for HIMB was made possible by a generous donation from Pam Omidyar (financing provided through the School of Ocean and Earth Science and Technology).

Microscope Applications

The Zeiss LSM 710 Confocal Laser Scanning Microscope system has a multitude of functions for fluorescent imaging and analysis. Some of the features of this system include the following:

     - Colocalization of fluorescent components
     - Fluorescence intensity measurements for any region of interest
     - Z-Sectioning of specimens for orthogonal & 3D analysis
     - Time lapse imaging of dynamic processes in living specimens
     - Fluorescence Resonance Energy Transfer (FRET)
     - Fluorescence Recovery After Photobleaching (FRAP)
     - Spectral imaging for the separation of overlapping emission spectra of specimens labeled with more than one fluorescent
        dye or expressing more than one innate fluorescent protein.

Objectives Available

N.A

W.D.
(mm)

Coverslip
thickness (mm)

Plan-Apochromat, 63X Oil DIC M27

1.4

0.19

0.17

C-Apochromat, 63X W Corr M27

1.2

0.28

0.17

EC Plan-NEOFLUAR 40X Oil DIC

1.3

0.21

0.17

C-Apochromat, 40X W Corr M27

1.2

0.28

0.17

Plan-Apochromat, 20X M27

0.8

0.55

0.17

C-Apochromat, 10X W M27

0.45

1.8

0.17

Contact Information

Location:

Pauley Research Bldg., Research Module I, rm 106

Phone:

(808) 236-7462

Email:

aeggers@hawaii.edu

Hours:

9:00 AM to 5:00 PM, Monday through Friday(after hours access available upon request)

Director:

Ruth Gates, Ph.D.

Manager:

Amy Eggers, MB(ASCP)

Reservation and Rates

Please request time on the instrument by contacting aeggers@hawaii.edu.

The user fees are designed to help support the service contract and, in the future, to support remote use and educational outreach in conjunction with Pam Omidyar of the Omidyar Network. Fees will be billed to recharge accounts on a monthly basis. Bulk rates are available for those using of the scope over 100 hours per year.

Outside investigators who would like to use the facility are welcome to do so for the same fee schedule, however, arrangements must be made for scheduling, billing, and payment prior to using the facility by contacting Amy Eggers at aeggers@hawaii.edu.

Description

Fees
(min. charge = 1 hr)

Certified independent user

$25/hr

Scanning by a technologist

$50/hr

Useful links:

1) Confocal Facility Policies

2) LSM 710 Operation Manual

3) Coral Under Confocal Video

4) The Microscopic World of Corals Video

 

[Top of the page]